DNA stretching on functionalized gold surfaces. [Nucleic Acids Res. 1994]

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1: Nucleic Acids Res. 1994 Feb 11;22(3):492-7. Links

DNA stretching on functionalized gold surfaces.

Zimmermann RM, Cox EC.

Department of Molecular Biology, Princeton University, NJ 08544.

We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.

PMID: 8127690 [PubMed - indexed for MEDLINE]

PMCID: PMC523609

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DNA stretching on functionalized gold surfaces.

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