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1: Nippon Jinzo Gakkai Shi. 1994 Jan;36(1):9-12.Links

Identification of GTP-binding proteins in human glomeruli.

Nitta K, Uchida K, Kawashima A, Tsutsui T, Ozu H, Naito T, Yumura W, Nihei H.

Department of Medicine, Kidney Center, Tokyo Women's Medical College, Japan.

The localization of GTP-binding proteins (G-proteins) in human glomeruli was examined using immunohistochemistry and immunoblotting. Immunohistochemical staining for G-protein subunits demonstrated the existence of Gs alpha, Gi alpha and Go alpha proteins in the glomeruli. Moreover, immunoblots further revealed Gs alpha (52 kD), Gi alpha 1/2 (40-41 kD), Gi alpha 3 (40 kD) and Go alpha (39 kD) in the glomerular membranes. The predominant subspecies of Gs was a 52-kD protein, and Go alpha was detectable in the smallest amounts of the G-protein subunits. However, immunoblots failed to demonstrate detectable amounts of G-proteins in cytosolic extracts. This is the first report that characterizes G-protein subunits in human glomeruli. Further study is required to determine the roles of G-proteins in signal transductions in human glomeruli.

PMID: 8107314 [PubMed - indexed for MEDLINE]