Abstract
We cloned a cDNA for the rat kappa-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [3H]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by kappa-specific opioid ligands, but not by mu- and delta-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that kappa-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.
MeSH terms
-
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
-
Amino Acid Sequence
-
Analgesics / pharmacology
-
Animals
-
Base Sequence
-
Cells, Cultured
-
Cloning, Molecular
-
DNA
-
Dynorphins / pharmacology
-
Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
-
Enkephalin, D-Penicillamine (2,5)-
-
Enkephalins / pharmacology
-
Molecular Sequence Data
-
Pyrrolidines / pharmacology
-
Rats
-
Receptors, Opioid, delta / genetics
-
Receptors, Opioid, kappa / drug effects
-
Receptors, Opioid, kappa / genetics*
-
Sequence Homology, Amino Acid
Substances
-
Analgesics
-
Enkephalins
-
Pyrrolidines
-
Receptors, Opioid, delta
-
Receptors, Opioid, kappa
-
Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
-
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer
-
Dynorphins
-
Enkephalin, D-Penicillamine (2,5)-
-
DNA