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1: Int J Radiat Biol. 1993 Jun;63(6):743-7.Links

Evidence for formation of somatic cell hybrids in a population of irradiated cells.

Andrushkevich VV, Kalendo GS, Semenyak OJu , Kakpakova ES.

Cancer Research Center of RAMS, Moscow, Russia.

Experiments using two genetically marked lines of Djungarian hamster cells (DM-15 HPRT- and DH-TK-) and the technique of hybrid selection in selective HAT medium revealed viable colonies in a mixed culture irradiated with a dose of 5 Gy. The sublines grown from these colonies were examined. Chromosome analysis showed that about 45% of those cells were hybrids inheriting chromosome markers of both parent strains. Formation of radio-induced hybrids occurs as a function of time after irradiation, 6 days proving to be the optimal interval. It is postulated that radiation-induced cell fusion and formation of viable somatic cell hybrids may be essential for cell population survival in the course of tumour radiotherapy.

PMID: 8100261 [PubMed - indexed for MEDLINE]

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