Cloning and sequencing of the cDNAs encoding two a...[Biochim Biophys Acta. 1994]

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1: Biochim Biophys Acta. 1994 Sep 8;1223(2):306-8. Links

Cloning and sequencing of the cDNAs encoding two alternative splicing-derived variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor.

Hu X, Emanuel PD, Zuckerman KS.

Division of Medical Oncology and Hematology, University of South Florida, Tampa 33612.

Two distinct cDNA clones, corresponding to alternative mRNA splicing variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor (GM-CSF-R alpha) were isolated from human blood mononuclear cells by RT-PCR. The first one lacks 179 bp, and the second one lacks 136 bp of the major variant, GM-CSF-R alpha 1. Both of variants result in shifts in the reading frame and are expected to encode 377- and 285-amino-acid membrane anchoring and soluble receptor isoforms, respectively.

PMID: 8086503 [PubMed - indexed for MEDLINE]

Cloning and sequencing of an alternative splicing-derived cDNA variant of the GM-CSF receptor alpha subunit, which encodes a truncated protein.
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[Curr Pharm Des. 2002]
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Cloning and sequencing of the cDNAs encoding two alternative splicing-derived variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor.

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