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Biochim Biophys Acta. 1994 Sep 13;1219(1):157-9.

Instability of human TATA-binding protein CAG triplet repeats during amplification by PCR.

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  • 1Laboratory for Physiological Chemistry, Utrecht University, The Netherlands.


Polymerase chain reaction (PCR) of a TATA-binding protein cDNA that contains CAG triplet repeats results in heterogeneous products. This is caused by a variable loss in the number of CAG triplets. Sequence analysis of PCR products suggests that instability increases with repeat length.

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