An interleukin-4-induced transcription factor: IL-4 Stat

Science. 1994 Sep 16;265(5179):1701-6. doi: 10.1126/science.8085155.

Abstract

Interleukin-4 (IL-4) is an immunomodulatory cytokine secreted by activated T lymphocytes, basophils, and mast cells. It plays an important role in modulating the balance of T helper (Th) cell subsets, favoring expansion of the Th2 lineage relative to Th1. Imbalance of these T lymphocyte subsets has been implicated in immunological diseases including allergy, inflammation, and autoimmune disease. IL-4 may mediate its biological effects, at least in part, by activating a tyrosine-phosphorylated DNA binding protein. This protein has now been purified and its encoding gene cloned. Examination of the primary amino acid sequence of this protein indicates that it is a member of the signal transducers and activators of transcription (Stat) family of DNA binding proteins, hereby designated IL-4 Stat. Study of the inhibitory activities of phosphotyrosine-containing peptides derived from the intracellular domain of the IL-4 receptor provided evidence for direct coupling of receptor and transcription factor during the IL-4 Stat activation cycle. Such observations indicate that IL-4 Stat has the same functional domain for both receptor coupling and dimerization.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • Cross-Linking Reagents
  • DNA / metabolism
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Interleukin-4 / pharmacology*
  • Interleukin-4 Receptor alpha Subunit
  • Models, Biological
  • Molecular Sequence Data
  • Monocytes / metabolism
  • Phosphopeptides / metabolism
  • Phosphopeptides / pharmacology
  • Phosphorylation
  • Polymers
  • Receptors, Cell Surface
  • Receptors, Interleukin-4
  • Receptors, Mitogen / metabolism*
  • STAT6 Transcription Factor
  • Trans-Activators / chemistry
  • Trans-Activators / genetics
  • Trans-Activators / isolation & purification
  • Trans-Activators / metabolism*
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Cross-Linking Reagents
  • DNA-Binding Proteins
  • IL4R protein, human
  • Interleukin-4 Receptor alpha Subunit
  • Phosphopeptides
  • Polymers
  • Receptors, Cell Surface
  • Receptors, Interleukin-4
  • Receptors, Mitogen
  • STAT6 Transcription Factor
  • STAT6 protein, human
  • Trans-Activators
  • Transcription Factors
  • Interleukin-4
  • DNA

Associated data

  • GENBANK/U16031