Display Settings:

Format

Send to:

Choose Destination
Gene. 1994 Sep 2;146(2):273-8.

Structure and expression of an inducible HSP70-encoding gene from Mus musculus.

Author information

  • 1Department of Biochemistry, Faculty of Medicine, University of Toronto, Ontario, Canada.

Abstract

We have determined the nucleotide sequence of a stress-inducible mouse Hsp70-encoding gene named hsp70A1. The gene encodes a 641-amino-acid protein whose deduced sequence is similar to those of other members of the HSP70 family. The 5' end (tsp) of a heat-inducible mRNA is 225 bp upstream from the start codon, and several consensus recognition sequences for transcription factors lie upstream from this tsp. There are 17 putative binding sites for heat-shock transcription factor (HSF), including three clusters of multiple binding sites. We show that this upstream region is sufficient to direct heat-inducible expression of a hsp70A1::cat hybrid gene in mouse and human cells.

PMID:
8076831
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk