HNF-3 beta is essential for node and notochord formation in mouse development

Cell. 1994 Aug 26;78(4):561-74. doi: 10.1016/0092-8674(94)90522-3.

Abstract

HNF-3 beta, a member of the HNF-3/fork head family of transcription factors, is expressed in the node, notochord, floor plate, and gut in mouse embryos. A null mutation of this gene leads to embryonic lethality. The primary defect of HNF-3 beta -/- embryos is an absence of organized node and notochord formation, which leads to secondary defects in dorsal-ventral patterning of the neural tube. In contrast, patterning along the anterior-posterior axis was surprisingly little affected. Although HNF-3 beta is required for node and notochord formation, some organizer activity persists in the absence of these structures. HNF-3 beta is not required for the development of definitive endoderm cells, but foregut morphogenesis is severely affected in HNF-3 beta -/- embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Central Nervous System / embryology*
  • Chimera
  • Crosses, Genetic
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Digestive System / embryology
  • Embryonic and Fetal Development*
  • Female
  • Gastrula / physiology
  • Genes, Lethal
  • Hepatocyte Nuclear Factor 3-beta
  • Male
  • Mesoderm / chemistry
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Notochord / embryology*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / physiology*
  • Phenotype
  • Transcription Factors / genetics
  • Transcription Factors / physiology*

Substances

  • DNA-Binding Proteins
  • Foxa2 protein, mouse
  • Nuclear Proteins
  • Transcription Factors
  • Hepatocyte Nuclear Factor 3-beta