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Gene. 1994 Aug 19;146(1):87-9.

Sequence of hcy, a gene encoding cytochrome c-554 from Nitrosomonas europaea.

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  • 1Laboratory for Nitrogen Fixation Research, Oregon State University, Corvallis 97331-2902.


Cytochrome c-554 (Cyt c-554) was purified from Nitrosomonas europaea. The N-terminal and internal amino acid sequences were determined. A synthetic oligodeoxyribonucleotide primer based on the N-terminal sequence was used to construct a PCR clone. This clone was used to identify genomic DNA fragments containing the gene encoding Cyt c-554. We determined the nucleotide sequence of this gene and named it hcy for hydroxylamine oxidoreductase-linked cytochrome.

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