Respiratory effects of single positive and negative pressure pulses (PPP, NPP) applied to the functionally isolated upper airways (UA) were studied in 11 anaesthetized cats breathing spontaneously through a tracheal tube. The UA pressure and the changes of tracheal airflow were recorded and the blood pressure and electrocardiogram were occasionally monitored. Sniff-like aspiration reflexes comprising powerful spasmodic inspirations could be elicited by PPP or NPP of 20 to 110 cm H2O or -14 to -140 cm H2O. The responses to NPP but also to PPP characterized by high peak inspiratory flow, mean inspiratory flow and tidal volume (PIF = 312.5 +/- 64.3 and 231.1 +/- 21.7 ml.sec-1; VTI = 178.3 +/- 46.7 and 110.1 +/- 14.4 ml.sec-1; VT = 40.9 +/- 8.3 and 22.5 +/- 1.7 ml) resembled closely the aspiration reflex elicited by mechanical stimulation of the pharyngeal wall. Occasionally, sneezing, minor modifications of breathing pattern and solitary forced inspirations could be induced by lower pressures. The results indicate that sudden pressure stimulation of the UA evokes vigorous respiratory responses including the aspiration reflex. These reflexes and their alterations may contribute to development or release of both UA obstruction and apnoea, at least in cats.