Am J Clin Pathol. 1975 Feb;63(2):237-9.

A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose.

Abstract

A method for the separation of lymphocytes from human blood by Ficoll-Hypaque density centrifugation, with removal of platelets by a single sucrose solution with a density of 65%, is presented. The Ficoll-Hypaque-sucrose method gives a good yield of lymphocytes, high viability and purity, and relatively low contamination with erythrocytes and platelets.

PMID:: 803775; [PubMed - indexed for MEDLINE]

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[Effect of anticoagulants on lymphocyte isolation by density gradient centrifugation]. [Acta Haematol Pol. 1981]
[Effect of anticoagulants on lymphocyte isolation by density gradient centrifugation].
Gnojkowski J, Klimek D, Chmiel J, Tabaka K. Acta Haematol Pol. 1981 Apr-Jun; 12(2):77-83.
A rapid method for isolation of human mononuclear cells free of significant platelet contamination. [J Immunol Methods. 1982]
A rapid method for isolation of human mononuclear cells free of significant platelet contamination.
Casale TB, Kaliner M. J Immunol Methods. 1982 Dec 30; 55(3):347-53.
A simple method for the isolation of platelet-free lymphocyte suspensions from rat whole blood. [J Immunol Methods. 1980]
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Hewitt CW, Sawyer S, Beck PA, Martin DC. J Immunol Methods. 1980; 36(3-4):227-34.
Isolation of human peripheral blood lymphocytes: modification of a double discontinuous density gradient of Ficoll-Hypaque. [J Immunol Methods. 1977]
Isolation of human peripheral blood lymphocytes: modification of a double discontinuous density gradient of Ficoll-Hypaque.
Goldrosen MH, Gannon PJ, Lutz M, Holyoke ED. J Immunol Methods. 1977; 14(1):15-7.
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Separation of human lymphocytes from citrated blood by density gradient (NycoPrep) centrifugation: monocyte depletion depending upon activation of membrane potassium channels.
Bøyum A, Brincker Fjerdingstad H, Martinsen I, Lea T, Løvhaug D. Scand J Immunol. 2002 Jul; 56(1):76-84.

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A simple method for the elimination of platelets from the lymphocyte-platelet mixture by sucrose.

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