Display Settings:

Format

Send to:

Choose Destination
We are sorry, but NCBI web applications do not support your browser and may not function properly. More information
FEBS Lett. 1994 Jul 18;348(3):278-82.

Isolation of a potassium-selective ion channel from the plasma membrane of the broad bean Vicia faba L.

Author information

  • Institut für Biophysik, Universität Hannover, Germany.

Abstract

Potassium ions are essential for plant nutrition and play an important role in osmoregulation and plant movement. Recent patch clamp investigations and molecular analysis have identified voltage-dependent potassium channels responsible for translocation of this ion across plant membranes. In order to characterize these transporters on the biochemical level, a potassium channel protein was isolated from Vicia faba under preservation of its functional integrity. Potassium channel activity was monitored by tracer flux and black lipid bilayer measurements. The capability for K+ transport copurified with a 67 kDa protein from V. faba plasma membranes. A monoclonal antibody directed against the 67 kDa protein blocked channel activity. Single channels with a unit conductance of 40 pS were highly selective for K+ over Na+.

PMID:
8034054
[PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk