Format

Send to:

Choose Destination
See comment in PubMed Commons below
Plant Physiol. 1994 May;105(1):269-77.

Expression of lipoxygenase in wounded tubers of Solanum tuberosum L.

Author information

  • 1Max-Planck-Institut für Züchtungsforschung, Abteilung Genetische Grundlagen der Pflanzenzüchtung, Köln, Germany.

Abstract

A lipoxygenase cDNA clone from Solanum tuberosum L. was analyzed to study the role of lipoxygenases in potato development and wound response. Sequence analysis and comparison of the deduced amino acid sequence revealed high homology to other plant lipoxygenases. Expression of the cDNA sequences in Escherichia coli and subsequent analysis of bacterial protein extracts showed lipoxygenase activity using linoleic, linolenic, or arachidonic acid as substrates. Transcripts encoding the potato lipoxygenase were most abundant in tuber tissue, lower in roots, and hardly detectable in leaves, petioles, and stems. The induction of lipoxygenase expression in tubers by wounding was dependent on various parameters. Whereas lipoxygenase transcript levels increased in discs from stored tubers incubated under aerobic conditions, tubers taken from a growing plant did not accumulate lipoxygenase transcripts in response to wounding. Incubation of tuber discs in buffer did not lead to an increase in lipoxygenase RNA levels; however, methyl jasmonate stimulated lipoxygenase expression after 24 h in stored tubers. Proteinase inhibitor II mRNAs decreased in stored tubers as well as in discs from growing tubers.

PMID:
8029354
[PubMed - indexed for MEDLINE]
PMCID:
PMC159354
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk