J Clin Microbiol. 1994 Apr;32(4):1095-8.

Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas.

Kapur V, Li LL, Iordanescu S, Hamrick MR, Wanger A, Kreiswirth BN, Musser JM.

Source

Department of Pathology, Baylor College of Medicine, Houston, TX 77030.

Abstract

Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region and showed that most are missense mutations. The analysis also identified several mutant rpoB alleles not previously associated with resistant organisms and one short region of rpoB that had an unusually high frequency of insertions and deletions. Although many strains with an identical IS6110 restriction fragment length polymorphism pattern have the same variant rpoB allele, some do not, a result that suggests the occurrence of evolutionary divergence at the clone level.

PMID:: 8027320; [PubMed - indexed for MEDLINE]
PMCID:: PMC267194

Free PMC Article

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

RIFAMPIN - HSDB

Supplemental Content

Save items

Related citations in PubMed

Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from the Middle East. [Diagn Microbiol Infect Dis. 2000]
Characterization of rpoB mutations in rifampin-resistant Mycobacterium tuberculosis isolates from the Middle East.
Ahmad S, Araj GF, Akbar PK, Fares E, Chugh TD, Mustafa AS. Diagn Microbiol Infect Dis. 2000 Dec; 38(4):227-32.
Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait and Dubai. [Diagn Microbiol Infect Dis. 2002]
Characterization of rpoB mutations in rifampin-resistant clinical Mycobacterium tuberculosis isolates from Kuwait and Dubai.
Ahmad S, Mokaddas E, Fares E. Diagn Microbiol Infect Dis. 2002 Nov; 44(3):245-52.
Rifampicin resistance and mutation of the rpoB gene in Mycobacterium tuberculosis. [FEMS Microbiol Lett. 1996]
Rifampicin resistance and mutation of the rpoB gene in Mycobacterium tuberculosis.
Taniguchi H, Aramaki H, Nikaido Y, Mizuguchi Y, Nakamura M, Koga T, Yoshida S. FEMS Microbiol Lett. 1996 Oct 15; 144(1):103-8.
Detection of rifampin resistance among isolates of Mycobacterium tuberculosis from Mozambique. [Microb Drug Resist. 1995]
Detection of rifampin resistance among isolates of Mycobacterium tuberculosis from Mozambique.
Caugant DA, Sandven P, Eng J, Jeque JT, Tønjum T. Microb Drug Resist. 1995 Winter; 1(4):321-6.
Independent origin of mono-rifampin-resistant Mycobacterium tuberculosis in patients with AIDS. [Am J Respir Crit Care Med. 1996]
Independent origin of mono-rifampin-resistant Mycobacterium tuberculosis in patients with AIDS.
Lutfey M, Della-Latta P, Kapur V, Palumbo LA, Gurner D, Stotzky G, Brudney K, Dobkin J, Moss A, Musser JM, et al. Am J Respir Crit Care Med. 1996 Feb; 153(2):837-40.

See reviews... See all...

Cited by 81 PubMed Central articles

Drug resistance mutations and heteroresistance detected using the GenoType MTBDRplus assay and their implication for treatment outcomes in patients from Mumbai, India. [BMC Infect Dis. 2012]
Drug resistance mutations and heteroresistance detected using the GenoType MTBDRplus assay and their implication for treatment outcomes in patients from Mumbai, India.
Tolani MP, D'souza DT, Mistry NF. BMC Infect Dis. 2012 Jan 20; 12:9. Epub 2012 Jan 20.
Transmission of dapsone-resistant leprosy detected by molecular epidemiological approaches. [Antimicrob Agents Chemother. 2...]
Transmission of dapsone-resistant leprosy detected by molecular epidemiological approaches.
Li W, Sakamuri RM, Lyons DE, Orcullo FM, Shinde V, Dela Pena EL, Maghanoy AA, Mallari IB, Tan EV, Nath I, et al. Antimicrob Agents Chemother. 2011 Nov; 55(11):5384-7. Epub 2011 Aug 22.
Rapid Detection of Mutation in RRDR of rpo B Gene for Rifampicin Resistance in MDR-Pulmonary Tuberculosis by DNA Sequencing. [Indian J Clin Biochem. 2010]
Rapid Detection of Mutation in RRDR of rpo B Gene for Rifampicin Resistance in MDR-Pulmonary Tuberculosis by DNA Sequencing.
Patra SK, Jain A, Sherwal BL, Khanna A. Indian J Clin Biochem. 2010 Jul; 25(3):315-8. Epub 2010 Aug 25.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Characterization by automated DNA sequencing of mutations in the gene (rpoB) enc...
Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas.
J Clin Microbiol. 1994 Apr ;32(4):1095-8.

PubMed
Point mutation of Arg440 to His in cytochrome P450c17 causes severe 17 alpha-hyd...
Point mutation of Arg440 to His in cytochrome P450c17 causes severe 17 alpha-hydroxylase deficiency.
J Clin Endocrinol Metab. 1994 Jul ;79(1):160-4.

PubMed
Differential phosphorylation of intracellular domains of gamma-aminobutyric acid...
Differential phosphorylation of intracellular domains of gamma-aminobutyric acid type A receptor subunits by calcium/calmodulin type 2-dependent protein kinase and cGMP-dependent protein kinase.
J Biol Chem. 1994 Jul 8 ;269(27):18111-7.

PubMed
Twin resemblance in somatotype and comparisons with other twin studies.
Twin resemblance in somatotype and comparisons with other twin studies.
Hum Biol. 1994 Jun ;66(3):453-64.

PubMed
Cloning and expression of a novel form of leukotriene B4 omega-hydroxylase from ...
Cloning and expression of a novel form of leukotriene B4 omega-hydroxylase from human liver.
FEBS Lett. 1994 Jul 4 ;348(1):70-4.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase beta subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas.

Source

Abstract

MeSH Terms, Substances, Secondary Source ID

MeSH Terms

Substances

Secondary Source ID

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

Supplemental Content

Save items

Related citations in PubMed

Cited by 81 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information