J Bacteriol. 1994 Jul;176(14):4416-23.

Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.

Menon NK, Chatelus CY, Dervartanian M, Wendt JC, Shanmugam KT, Peck HD Jr, Przybyla AE.

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Department of Biochemistry, University of Georgia, Athens 30602.

Abstract

The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.

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