Inactivation of the Podospora anserina vegetative incompatibility locus het-c, whose product resembles a glycolipid transfer protein, drastically impairs ascospore production

S Saupe; C Descamps; B Turcq; J Bégueret

doi:10.1073/pnas.91.13.5927

Inactivation of the Podospora anserina vegetative incompatibility locus het-c, whose product resembles a glycolipid transfer protein, drastically impairs ascospore production

Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):5927-31. doi: 10.1073/pnas.91.13.5927.

Authors

S Saupe¹, C Descamps, B Turcq, J Bégueret

Affiliation

¹ Laboratoire de Génétique, UPR 9026 Centre National de la Recherche Scientifique, Université de Bordeaux II, Talence, France.

Abstract

The het-c locus contains different alleles that elicit nonallelic vegetative incompatibility through specific interactions with alleles of the unlinked loci het-e and het-d. The het-c2 allele has been cloned. It encodes a 208-amino acid polypeptide that is similar to a glycolipid transfer protein purified from pig brain. Disruption of this gene drastically impairs ascospore production in homozygous crosses, and some mutants exhibit abnormal branching of apical hyphae. The protein encoded by het-c2 is essential in the biology of the fungus and may be involved in cell-wall biosynthesis.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Amino Acid Sequence
Ascomycota / genetics*
Ascomycota / physiology
Base Sequence
Carrier Proteins / biosynthesis
Carrier Proteins / genetics*
Crosses, Genetic
DNA Primers
Fungal Proteins / biosynthesis
Fungal Proteins / genetics*
Fungal Proteins / isolation & purification
Genes, Fungal*
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Homology, Amino Acid
Spores, Fungal / physiology

Substances

Carrier Proteins
DNA Primers
Fungal Proteins
HET-C2 protein, Podospora anserina
lipid transfer protein

Associated data

GENBANK/U05236