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Cloning and expression of recombinant human growth/differentiation factor 5.
Biopharm GmbH, Heidelberg, Germany.
The complete amino acid sequence of human Growth/differentiation factor 5 (huGdf5), a new member of the TGF-beta superfamily, has been determined through initial degenerate PCR and subsequent cloning and nucleotide sequencing of genomic DNA and cDNA encoding the precursor and flanking regions. The huGdf5 gene consists of only two coding exons. The protein is highly homologous to its murine equivalent, particularly the mature part which differs only by one amino acid. Expression in HuTK- cells using recombinant vaccinia virus revealed the expected processed dimeric mature protein. Antibodies against huGdf5 were raised in chicken.
PMID: 7980526 [PubMed - indexed for MEDLINE]
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Cited by 3 PubMed Central articles
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Nerve growth factor modulation of the cavernous nerve response to injury.
Bella AJ, Lin G, Lin CS, Hickling DR, Morash C, Lue TF.
J Sex Med. 2009 Mar; 6 Suppl 3:347-52.
[J Sex Med. 2009]
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Cloning and functional characterization of the 5'-flanking region of the human bone morphogenetic protein-2 gene.
Sugiura T.
Biochem J. 1999 Mar 1; 338 ( Pt 2):433-40.
[Biochem J. 1999]
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Ectopic induction of tendon and ligament in rats by growth and differentiation factors 5, 6, and 7, members of the TGF-beta gene family.
Wolfman NM, Hattersley G, Cox K, Celeste AJ, Nelson R, Yamaji N, Dube JL, DiBlasio-Smith E, Nove J, Song JJ, et al.
J Clin Invest. 1997 Jul 15; 100(2):321-30.
[J Clin Invest. 1997]