Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Cell Biol. 1994 Dec;14(12):7884-90.

Replication factor A is required in vivo for DNA replication, repair, and recombination.

Author information

  • 1Dipartimento di Genetica e di Biologia dei Microrganismi, Universit√† di Milano, Italy.

Abstract

Replication factor A (RF-A) is a heterotrimeric single-stranded-DNA-binding protein which is conserved in all eukaryotes. Since the availability of conditional mutants is an essential step to define functions and interactions of RF-A in vivo, we have produced and characterized mutations in the RFA1 gene, encoding the p70 subunit of the complex in Saccharomyces cerevisiae. This analysis provides the first in vivo evidence that RF-A function is critical not only for DNA replication but also for efficient DNA repair and recombination. Moreover, genetic evidence indicate that p70 interacts both with the DNA polymerase alpha-primase complex and with DNA polymerase delta.

PMID:
7969128
[PubMed - indexed for MEDLINE]
PMCID:
PMC359327
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk