J Immunol Methods. 1976;13(3-4):261-70.

Immunohistochemical identification of T- and B-lymphocytes delineated by the unlabeled antibody enzyme method. I. Anatomical distribution of theta-positive and Ig-positive cells in lymphoid organs of mice.

Hoffmann-Fezer G, Rodt H, Eulitz M, Thierfelder S.

Abstract

The unlabeled antibody enzyme method was used to delineate the anatomical distribution of lymphocytes positive with a MBtheta and a-MIg in tissue sections of thymus, spleen, lymph nodes and Peyer's patches of mice. The known T- and B-cell areas were established. Moreover, individual B-cells are detected in T-cell regions, especially in the thymus medulla, and in the periarteriolar section of spleen white pulp. Similarly, individual T-cells occur in B-cell areas, namely in the marginal zone of spleen white pulp, in the medullar cores of lymph nodes, and in germinal centers.

PMID:: 796387; [PubMed - indexed for MEDLINE]

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Receptors, Antigen, B-Cell

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Immunohistochemical identification of T- and B-lymphocytes delineated by the unlabeled antibody enzyme method. I. Anatomical distribution of theta-positive and Ig-positive cells in lymphoid organs of mice.

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