A plasmid from the methylotrophic actinomycete Amycolatopsis methanolica capable of site-specific integration

J Bacteriol. 1994 Nov;176(22):7087-90. doi: 10.1128/jb.176.22.7087-7090.1994.

Abstract

Amycolatopsis methanolica contains a 13.3-kb plasmid (pMEA300) which is present both in the free state and integrated at a unique genomic location. A 2.1-kb pMEA300 DNA fragment was sequenced, revealing the putative attP site and two open reading frames, xis and int, showing similarity to genes encoding excisionases and integrases, respectively.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actinobacteria / genetics*
  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • DNA Nucleotidyltransferases / genetics
  • DNA Transposable Elements / genetics*
  • Integrases
  • Methanol / metabolism
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Recombination, Genetic / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Viral Proteins*

Substances

  • DNA Transposable Elements
  • Viral Proteins
  • DNA Nucleotidyltransferases
  • Integrases
  • excisionase
  • Methanol

Associated data

  • GENBANK/L36679