FEBS Lett. 1994 Nov 21;355(1):106-8.

Brazzein, a new high-potency thermostable sweet protein from Pentadiplandra brazzeana B.

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Department of Animal Health and Biomedical Sciences, University of Wisconsin at Madison, 53706.

Abstract

We have discovered a new high-potency thermostable sweet protein, which we name brazzein, in a wild African plant Pentadiplandra brazzeana Baillon. Brazzein is 2,000 times sweeter than sucrose in comparison to 2% sucrose aqueous solution and 500 times in comparison to 10% of the sugar. Its taste is more similar to sucrose than that of thaumatin. Its sweetness is not destroyed by 80 degrees C for 4 h. Brazzein is comprised of 54 amino acid residues, corresponding to a molecular mass of 6,473 Da.

PMID:: 7957951; [PubMed - indexed for MEDLINE]

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Cited by 6 PubMed Central articles

Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein. [PLoS One. 2011]
Identification and modulation of the key amino acid residue responsible for the pH sensitivity of neoculin, a taste-modifying protein.
Nakajima K, Yokoyama K, Koizumi T, Koizumi A, Asakura T, Terada T, Masuda K, Ito K, Shimizu-Ibuka A, Misaka T, et al. PLoS One. 2011 Apr 29; 6(4):e19448. Epub 2011 Apr 29.
Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor. [J Mol Biol. 2010]
Key amino acid residues involved in multi-point binding interactions between brazzein, a sweet protein, and the T1R2-T1R3 human sweet receptor.
Assadi-Porter FM, Maillet EL, Radek JT, Quijada J, Markley JL, Max M. J Mol Biol. 2010 May 14; 398(4):584-99. Epub 2010 Mar 17.
Design and evaluation of new analogs of the sweet protein brazzein. [Chem Senses. 2009]
Design and evaluation of new analogs of the sweet protein brazzein.
Walters DE, Cragin T, Jin Z, Rumbley JN, Hellekant G. Chem Senses. 2009 Oct; 34(8):679-83. Epub 2009 Aug 20.

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Structures reported by this article

Solution Nmr Structure Of The Sweet Protein Brazzein, Minimized Average Structure

PDB: 2BRZ

Source: Pentadiplandra brazzeana

Method: Solution Nmr

See all 2 structures...

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