Abstract
The bchM gene of Rhodobacter sphaeroides has been sequenced and then overexpressed in E. coli producing a protein of M(r) approximately. 27,500. Cell-free extracts of the transformed E. coli strain are able to methylate added Mg protoporphyrin, resulting in the formation of Mg protoporphyrin monomethyl ester. The identity of this product was verified by HPLC. The bchM gene product is therefore assigned to the methyltransferase step in bacteriochlorophyll biosynthesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacteriochlorophylls / biosynthesis*
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Base Sequence
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Cloning, Molecular
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Escherichia coli / genetics
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Gene Expression
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Genes, Bacterial / genetics*
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Methylation
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Methyltransferases / chemistry
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Methyltransferases / genetics*
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Methyltransferases / metabolism
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Molecular Sequence Data
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Molecular Weight
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Rhodobacter sphaeroides / enzymology
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Rhodobacter sphaeroides / genetics*
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Sequence Analysis, DNA
Substances
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Bacteriochlorophylls
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Methyltransferases
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magnesium-protoporphyrin methyltransferase