Abstract
Functional analysis of T cells from the bronchial mucosa has been limited by difficulties in extracting T cells from this tissue. Because interleukin-2 (IL-2) is chemotactic for T cells, we determined whether this cytokine could be used to extract T cells from human bronchial wall (BW). Fresh tissue was obtained from 21 patients undergoing surgery for malignancy. Within the BW, 95% of T cells stained for the memory/activation marker CD45RO. When BW sections were incubated with IL-2 for 24 h, 88 to 91% of T cells emigrated into the culture medium. Compared with autologous blood T cells (also exposed to IL-2), these BW T cells expressed CD2 at a greater intensity and showed a fourfold reduction in cloning efficiency in response to phytohemagglutinin, and T-cell clones derived from the BW population displayed a tendency for higher interferon-gamma production. Furthermore, we were also able to extract and clone T cells from bronchoscopic biopsies in four subjects, suggesting that this method will provide a new avenue for examining T-cell function in airway inflammatory diseases.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Antigens, Differentiation, T-Lymphocyte / analysis
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Antigens, Surface / analysis
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Asthma / genetics
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Asthma / immunology*
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Asthma / pathology*
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Biopsy
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Bronchi / immunology
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Bronchi / metabolism
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Bronchi / pathology*
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Bronchoscopy
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CD2 Antigens
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CD3 Complex / analysis
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Carcinoma / pathology*
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Cell Separation / methods*
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Chemotaxis, Leukocyte
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Cloning, Molecular
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Evaluation Studies as Topic
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Gene Expression Regulation, Neoplastic*
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Humans
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Immunoenzyme Techniques
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Interferon-gamma / metabolism
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Interleukin-2*
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Interleukin-4 / metabolism
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Leukocyte Common Antigens / analysis
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Leukocyte Common Antigens / classification
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Lung Neoplasms / pathology*
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Middle Aged
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Mucous Membrane
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Phytohemagglutinins
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Receptors, Immunologic / analysis
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T-Lymphocytes / chemistry
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T-Lymphocytes / immunology
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T-Lymphocytes / metabolism
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T-Lymphocytes / pathology*
Substances
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Antigens, Differentiation, T-Lymphocyte
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Antigens, Surface
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CD2 Antigens
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CD3 Complex
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Interleukin-2
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Phytohemagglutinins
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Receptors, Immunologic
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Interleukin-4
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Interferon-gamma
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Leukocyte Common Antigens