Yeast. 1994 Oct;10(10):1355-61.

Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm.

Verhasselt P, Aert R, Voet M, Volckaert G.

Source

University of Leuven, Laboratory of Gene Technology, Belgium.

Abstract

The nucleotide sequence of 23.6 kb of the right arm of chromosome XIV is described, starting from the centromeric region. Both strands were sequenced with an average redundancy of 4.87 per base pair. The overall G+C content is 38.8% (42.5% for putative coding regions versus 29.4% for non-coding regions). Twelve open reading frames (ORFs) greater than 100 amino acids were detected. Codon frequencies of the twelve ORFs agree with codon usage in Saccharomyces cerevisiae and all show the characteristics of low level expressed genes. Five ORFs (N2019, N2029, N2031, N2048 and N2050) are encoded by previously sequenced genes (the mitochondrial citrate synthase gene, FUN34, RPC34, PRP2 and URK1, respectively). ORF N2052 shows the characteristics of a transmembrane protein. Other elements in this region are a tRNA(Pro) gene, a tRNA(Asn) gene, a tau 34 and a truncated delta 34 element. Nucleotide sequence comparison results in relocation of the SIS1 gene to the left arm of the chromosome as confirmed by colinearity analysis.

PMID:: 7900425; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

DNA, Fungal

Secondary Source ID

GENBANK/X77395

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Saccharomyces Genome Database

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online strain list - StrainInfo

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Nucleotide sequence analysis of an 8887 bp region of the left arm of yeast chromosome XIV, encompassing the centromere sequence. [Yeast. 1994]
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Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
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Cited in PMC
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Twelve open reading frames revealed in the 23.6 kb segment flanking the centrome...
Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm.
Yeast. 1994 Oct ;10(10):1355-61.

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