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Electrophoresis. 1994 Nov;15(11):1459-65.

The human myocardial two-dimensional gel protein database: update 1994.

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  • 1Department of Cardiothoracic Surgery, National Heart and Lung Institute, Heart Science Centre, Middlesex, UK.

Abstract

An updated human heart protein two-dimensional electrophoresis (2-DE) database is presented. The database, which contains some 1388 protein spots characterised in terms of M(r) and pI, has been analysed further by Western immunoblotting and protein sequencing. From a total of 103 protein spots analysed, 49 have been identified by immunoblotting and 32 have been identified by protein sequencing. A further six proteins have tentatively been assigned by comparison with the human heart 2-DE protein database of Jungblut et al. (Electrophoresis) 1994, 15, 685-607). This database is being used in studies of alterations in protein expression in the diseased and transplanted human heart.

PMID:
7895732
[PubMed - indexed for MEDLINE]
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