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Mamm Genome. 1994 Nov;5(11):688-95.

Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR.

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  • 1National Institute for Medical Research, London, UK.

Abstract

We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome.

PMID:
7873878
[PubMed - indexed for MEDLINE]
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