Send to:

Choose Destination
See comment in PubMed Commons below
Methods Cell Biol. 1994;41:297-316.

Immunochemical quantitation of bromodeoxyuridine: application to cell-cycle kinetics.

Author information

  • 1Biology and Biotechnology Program, Lawrence Livermore National Laboratory, Livermore, California 94550.


We have described several laboratory procedures for the immunochemical staining of the halopyrimidines, BrdUrd and IdUrd, in cell suspensions for flow cytometry and a method for staining histological sections on slides. Halogenated pyrimidine quantitation allows cell-cycle parameters, including total cell-cycle time, phase durations, and growth fraction to be determined. We have presented some flow cytometric data to demonstrate the use of these methods in determining bivariate BrdUrd/DNA histograms with CHO cells and in kinetic studies with the brown Norway rat myeloid leukemia model.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Loading ...
    Write to the Help Desk