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Methods Cell Biol. 1994;41:297-316.

Immunochemical quantitation of bromodeoxyuridine: application to cell-cycle kinetics.

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  • 1Biology and Biotechnology Program, Lawrence Livermore National Laboratory, Livermore, California 94550.

Abstract

We have described several laboratory procedures for the immunochemical staining of the halopyrimidines, BrdUrd and IdUrd, in cell suspensions for flow cytometry and a method for staining histological sections on slides. Halogenated pyrimidine quantitation allows cell-cycle parameters, including total cell-cycle time, phase durations, and growth fraction to be determined. We have presented some flow cytometric data to demonstrate the use of these methods in determining bivariate BrdUrd/DNA histograms with CHO cells and in kinetic studies with the brown Norway rat myeloid leukemia model.

PMID:
7861968
[PubMed - indexed for MEDLINE]
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