Parasitol Res. 1994;80(7):569-74.

Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing.

Ibrahim ME, Hag-Ali M, el-Hassan AM, Theander TG, Kharazmi A.

Source

Center for Medical Parasitology, University of Copenhagen, Denmark.

Abstract

A total of 17 Leishmania isolates, 6 of them isolated from antimony-resistant patients, were collected in the Sudan and tested for their sensitivity to sodium stibogluconate (Pentostam) as promastigotes. Six of those isolates were tested as amastigotes infecting a murine macrophage cell line. The results indicated that the conventional promastigote screening assay did not correlate with the clinical picture, whereas the amastigote/macrophage system produced results that pertained to the in vivo responses to the drug. A laboratory-generated resistant strain of L. major was adapted to grow at a high concentration of Pentostam (1000 micrograms/ml) as promastigotes but was quite sensitive to the drug at much lower concentrations in the amastigote/(macrophage system (20 micrograms/ml), thus suggesting that Pentostam's inhibitory action is mediated through the macrophage rather than through a direct toxic effect exerted on the parasite.

PMID:: 7855121; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Antimony Sodium Gluconate

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Leishmania donovani: an in vitro study of antimony-resistant amphotericin B-sensitive isolates. [Exp Parasitol. 2006]
Leishmania donovani: an in vitro study of antimony-resistant amphotericin B-sensitive isolates.
Sharief AH, Gasim Khalil EA, Theander TG, Kharazmi A, Omer SA, Ibrahim ME. Exp Parasitol. 2006 Dec; 114(4):247-52. Epub 2006 May 23.
Pentostam induces resistance to antimony and the preservative chlorocresol in Leishmania donovani promastigotes and axenically grown amastigotes. [Antimicrob Agents Chemother. 1...]
Pentostam induces resistance to antimony and the preservative chlorocresol in Leishmania donovani promastigotes and axenically grown amastigotes.
Ephros M, Waldman E, Zilberstein D. Antimicrob Agents Chemother. 1997 May; 41(5):1064-8.
Stage-specific activity of pentavalent antimony against Leishmania donovani axenic amastigotes. [Antimicrob Agents Chemother. 1...]
Stage-specific activity of pentavalent antimony against Leishmania donovani axenic amastigotes.
Ephros M, Bitnun A, Shaked P, Waldman E, Zilberstein D. Antimicrob Agents Chemother. 1999 Feb; 43(2):278-82.
Review Drug resistance mechanisms in clinical isolates of Leishmania donovani. [Indian J Med Res. 2006]
Review Drug resistance mechanisms in clinical isolates of Leishmania donovani.
Singh N. Indian J Med Res. 2006 Mar; 123(3):411-22.
Review Interaction of Leishmania with the host macrophage. [Trends Parasitol. 2002]
Review Interaction of Leishmania with the host macrophage.
Handman E, Bullen DV. Trends Parasitol. 2002 Aug; 18(8):332-4.

See reviews... See all...

Cited by 11 PubMed Central articles

Inhibition of ABC transporters abolishes antimony resistance in Leishmania Infection. [Antimicrob Agents Chemother. 2...]
Inhibition of ABC transporters abolishes antimony resistance in Leishmania Infection.
Mookerjee Basu J, Mookerjee A, Banerjee R, Saha M, Singh S, Naskar K, Tripathy G, Sinha PK, Pandey K, Sundar S, et al. Antimicrob Agents Chemother. 2008 Mar; 52(3):1080-93. Epub 2007 Dec 3.
Sodium antimony gluconate induces generation of reactive oxygen species and nitric oxide via phosphoinositide 3-kinase and mitogen-activated protein kinase activation in Leishmania donovani-infected macrophages. [Antimicrob Agents Chemother. 2...]
Sodium antimony gluconate induces generation of reactive oxygen species and nitric oxide via phosphoinositide 3-kinase and mitogen-activated protein kinase activation in Leishmania donovani-infected macrophages.
Mookerjee Basu J, Mookerjee A, Sen P, Bhaumik S, Sen P, Banerjee S, Naskar K, Choudhuri SK, Saha B, Raha S, et al. Antimicrob Agents Chemother. 2006 May; 50(5):1788-97.
Unresponsiveness to Glucantime treatment in Iranian cutaneous leishmaniasis due to drug-resistant Leishmania tropica parasites. [PLoS Med. 2006]
Unresponsiveness to Glucantime treatment in Iranian cutaneous leishmaniasis due to drug-resistant Leishmania tropica parasites.
Hadighi R, Mohebali M, Boucher P, Hajjaran H, Khamesipour A, Ouellette M. PLoS Med. 2006 May; 3(5):e162. Epub 2006 Apr 18.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Leishmania resistant to sodium stibogluconate: drug-associated macrophage-depend...
Leishmania resistant to sodium stibogluconate: drug-associated macrophage-dependent killing.
Parasitol Res. 1994 ;80(7):569-74.

PubMed
Selective neuroanatomic abnormalities in Down's syndrome and their cognitive cor...
Selective neuroanatomic abnormalities in Down's syndrome and their cognitive correlates: evidence from MRI morphometry.
Neurology. 1995 Feb ;45(2):356-66.

PubMed
Neurobiology. La différence vive.
Neurobiology. La différence vive.
Nature. 1995 Feb 16 ;373(6515):561-2.

PubMed
A third transactivation function (AF3) of human progesterone receptors located i...
A third transactivation function (AF3) of human progesterone receptors located in the unique N-terminal segment of the B-isoform.
Mol Endocrinol. 1994 Oct ;8(10):1347-60.

PubMed
Sigma S-dependent growth-phase induction of the csgBA promoter in Escherichia co...
Sigma S-dependent growth-phase induction of the csgBA promoter in Escherichia coli can be achieved in vivo by sigma 70 in the absence of the nucleoid-associated protein H-NS.
Mol Microbiol. 1994 Sep ;13(6):1021-32.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: