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    Biochem Mol Biol Int. 1994 Oct;34(3):595-601.

    An immunodetection method for the quantitation of human acyl CoA:glycine N-acyltransferase in biological samples.

    Mawal YR, Qureshi IA.

    Source

    Centre de recherche, Hôpital Sainte-Justine, Montréal, Québec, Canada.

    Abstract

    An immunochemical method for quantitation of human acyl CoA:glycine N-acyltransferase (ACGNAT) has been developed. A 33 kD ovine ACGNAT was purified to homogeneity and was used to raise polyclonal antibodies in rabbits. These antibodies were then employed for developing a sensitive immunodiagnostic method as they cross-reacted with human ACGNAT. Using this methodology, a linear correlation was seen between specific activity and ACGNAT concentration in an ammonium sulphate precipitation fraction (40-60%) of hepatic tissues of children and adults of 4 h age to 11 year old (r = 0.99, n = 6).

    PMID:
    7833837
    [PubMed - indexed for MEDLINE]

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