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    Biochem Biophys Res Commun. 1994 Dec 30;205(3):1617-24.

    Molecular cloning and functional characterization of a human VIP receptor from SUP-T1 lymphoblasts.

    Svoboda M, Tastenoy M, Van Rampelbergh J, Goossens JF, De Neef P, Waelbroeck M, Robberecht P.

    Department of Biochemistry and Nutrition, Medical School, Université Libre de Bruxelles, Belgium.

    We have cloned and sequenced a cDNA isolated from a human SUP-T1 lymphoblast cell line library. It encoded a 457 amino acids protein having 87% identity with the rat PACAP type II, VIP2 receptor. Chinese hamster ovary (CHO) cells stably transfected with cloned cDNA expressed a specific binding of 125I[Acetyl-His1]PACAP-27. This binding was inhibited by GTP, and by the peptides helodermin, VIP, PACAP-27 and PACAP-38 that also stimulated adenylate cyclase activity. The order of potency was PACAP-38 > VIP > or = helodermin > or = PACAP-27. Comparison of the results in two cell lines expressing different receptor densities suggested that helodermin and PACAP-38 had a higher intrinsic activity than VIP and PACAP-27.

    PMID: 7811244 [PubMed - indexed for MEDLINE]

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