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Parasitol Res. 1994;80(6):474-7.

Intra- and interspecific polymorphisms of Leishmania donovani and L. tropica minicircle DNA.

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  • 1Abteilung für Infektions- und Tropenmedizin, Universität München, Germany.

Abstract

A pair of degenerate polymerase chain reaction (PCR) primers (LEI-1, TCG GAT CC[C,T] [G,C]TG GGT AGG GGC GT; LEI-2, ACG GAT CC[G,C] [G,C][A,C]C TAT [A,T]TT ACA CC) defining a 0.15-kb segment of Leishmania minicircle DNA was constructed. These primers amplified not only inter- but also intraspecifically polymorphic sequences. Individual sequences revealed a higher intraspecific than interspecific divergence. It is concluded that individual sequences are of limited relevance for species determination. In contrast, when a data base of 19 different sequences was analyzed in a dendrographic plot, an accurate species differentiation was feasible.

PMID:
7808997
[PubMed - indexed for MEDLINE]
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