beta-Amyloid(25-35) induces the production of interleukin-8 from human monocytes

J Neuroimmunol. 1995 Jun;59(1-2):29-33. doi: 10.1016/0165-5728(95)00021-s.

Abstract

In an effort to unravel some of the cellular actions of beta-amyloid protein (A beta), we investigated its effects on interleukin-8 (IL-8) production from human monocytes. Supernatants harvested from cultured monocytes stimulated with the neurotoxic fragment 25-35 of beta-amyloid [A beta(25-35)] contained significant amounts of IL-8. Northern blot analysis demonstrated that A beta(25-35) also induced IL-8 mRNA accumulation. The effect of A beta(25-35) on IL-8 mRNA accumulation and secretion was not mimicked by a scrambled A beta(25-35) peptide, and was not affected by polymyxin B sulphate, which, on the other hand, almost completely abolished the effect of lipopolysaccharide. Our results uncover a new biological action of beta-amyloid: that of stimulating the production of a chemokine from monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amyloid beta-Peptides / pharmacology*
  • Cells, Cultured
  • Humans
  • Interleukin-8 / biosynthesis*
  • Interleukin-8 / genetics
  • Molecular Sequence Data
  • Monocytes / metabolism*
  • Peptide Fragments / pharmacology*
  • RNA, Messenger / analysis

Substances

  • Amyloid beta-Peptides
  • Interleukin-8
  • Peptide Fragments
  • RNA, Messenger
  • amyloid beta-protein (25-35)