J Biol Chem. 1976 Jun 25;251(12):3716-9.

Selective inhibition of the dnase activity of the recBC enzyme by the DNA binding protein from Escherichia coli.

Abstract

In the presence of the Escherichia coli DNA binding protein, single-stranded DNA is resistant to both the endo- and exonucleolytic activities of the recBC DNase. Linear duplex DNA, on the other hand, is unwound at a normal rate, but converted to large, single-stranded fragments which are resistant to further hydrolysis. Therefore, in the presence of the binding protein and linear duplex DNA, the recBC enzyme acts not as a DNase, but primarily as an ATP-dependent unwinding enzyme, able to generate large, single-stranded material. Duplex circular DNA containing short, single-stranded gaps is also resistant to the hydrolysis in the presence of the binding protein.

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Escherichia coli recA protein protects single-stranded DNA or gapped duplex DNA from degradation by RecBC DNase. [J Biol Chem. 1981]
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