Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
J Cell Sci. 1995 Feb;108 ( Pt 2):635-44.

Lamin proteins form an internal nucleoskeleton as well as a peripheral lamina in human cells.

Author information

  • 1CRC Nuclear Structure and Function Research Group, Sir William Dunn School of Pathology, University of Oxford, UK.


The nuclear lamina forms a protein mesh that underlies the nuclear membrane. In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the nuclear periphery. We now show that they also form part of a diffuse skeleton that ramifies throughout the interior of the nucleus. Unlike their peripheral counterparts, these internal lamins are buried in dense chromatin and so are inaccessible to antibodies, but accessibility can be increased by removing chromatin. Knobs and nodes on an internal skeleton can then be immunolabelled using fluorescein- or gold-conjugated anti-lamin A antibodies. These results suggest that the lamins are misnamed as they are also found internally.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire
    Loading ...
    Write to the Help Desk