Sequencing and expression of sheep angiotensinogen cDNA

M Nagase; F Suzuki; A Fukamizu; N Takeda; K Takeuchi; K Murakami; Y Nakamura

doi:10.1271/bbb.58.1884

Sequencing and expression of sheep angiotensinogen cDNA

Biosci Biotechnol Biochem. 1994 Oct;58(10):1884-5. doi: 10.1271/bbb.58.1884.

Authors

M Nagase¹, F Suzuki, A Fukamizu, N Takeda, K Takeuchi, K Murakami, Y Nakamura

Affiliation

¹ United Graduate School of Agricultural Sciences, Gifu University, Japan.

PMID: 7765514
DOI: 10.1271/bbb.58.1884

Abstract

A sheep liver cDNA library was screened with a BstEII fragment of human angiotensinogen cDNA to obtain sheep angiotensinogen cDNA. The cDNA sequence showed that preangiotensinogen consisted of 452 amino acid residues (49 kDa) of angiotensinogen and 24 residues of a signal peptide. Recombinant sheep angiotensinogen (56 kDa) was expressed in COS-7 cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Angiotensinogen / biosynthesis*
Angiotensinogen / genetics*
Animals
Base Sequence
Blotting, Western
Cells, Cultured
DNA, Complementary / biosynthesis*
Genomic Library
Humans
Liver / metabolism
Molecular Sequence Data
Recombinant Proteins / biosynthesis
Recombinant Proteins / genetics
Sheep

Substances

DNA, Complementary
Recombinant Proteins
Angiotensinogen