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J Clin Microbiol. 1995 Mar;33(3):629-30.

Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin.

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  • 1Dipartimento di Scienze Mediche, Oncologiche e Radiologiche, Universit√† di Modena, Italy.

Abstract

Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).

PMID:
7751368
[PubMed - indexed for MEDLINE]
PMCID:
PMC228003
Free PMC Article
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