Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biochimie. 1994;76(12):1133-42.

Enzymatic mechanism of tRNA (m5U54)methyltransferase.

Author information

  • 1Department of Pharmaceutical Chemistry, University of California, San Francisco 94143-0448, USA.

Abstract

tRNA (m5U54)methyltransferase (RUMT) catalyzes the methylation of uridine 54 of transfer RNA by S-adenosyl-L-methionine. In this report, we present the enzymatic mechanism of RUMT, including the stereochemical course of the methylation reaction, and discuss RUMT-tRNA recognition. As part of its enzymatic mechanism, we postulate that RUMT catalyzes the disruption of RNA-RNA contacts. We also show that many nucleotide substitutions can be made in the T-loop of tRNA without affecting RUMT binding, indicating that the recognition of the T-loop by RUMT is not stringent.

PMID:
7748948
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk