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1: Cytogenet Cell Genet. 1995;70(1-2):41-4.Links

Human origin of micronuclei in human x hamster two-cell embryos.

Tusell L, Alvarez R, Genescà A, Caballín MR, Miró R, Barrios L, Egozcue J.

Departament de Biologia Cellular i Fisiologia, Facultat de Medicina, Universitat Autònoma de Barcelona, Spain.

Using fluorescence in situ hybridization techniques with either human or hamster genomic DNA probes, we studied the origin of micronuclei in two-cell hybrid embryos obtained from hamster oocytes and gamma-irradiated human spermatozoa. Our study demonstrates that over 99% of micronuclei hybridize with human DNA probes and not with hamster DNA, revealing their human origin. Thus, the micronucleus test represents a good method to evaluate genetic damage in human germ cells, since it is simpler and faster than sperm chromosome studies.

PMID: 7736786 [PubMed - indexed for MEDLINE]