Suppression of human melanoma metastasis by introduction of chromosome 6 may be partially due to inhibition of motility, but not to inhibition of invasion

Biochem Biophys Res Commun. 1995 Mar 17;208(2):476-84. doi: 10.1006/bbrc.1995.1363.

Abstract

Active cellular motility and invasion play essential roles in metastasis. Introduction of normal, neo-tagged human chromosome 6 (neo6) into highly metastatic human melanoma cell line C8161 results in complete suppression of metastasis in vivo. To understand the mechanism by which metastasis was inhibited in neo6/C8161 hybrids, two in vitro assays, pseudopod protrusion and Membrane Invasion Culture System, were used to measure motility and invasion, respectively. neo6/C8161 hybrids are much less motile although they remained invasive, indicating that a metastasis-suppressor gene(s) on human chromosome 6 may regulate cellular motility, thereby inhibiting metastasis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Movement*
  • Chromosomes, Human, Pair 6
  • Collagen / metabolism
  • Drug Combinations
  • Endopeptidases / metabolism
  • Gene Transfer Techniques
  • Humans
  • In Vitro Techniques
  • Laminin / metabolism
  • Melanoma / pathology*
  • Neoplasm Invasiveness*
  • Neoplasm Metastasis*
  • Proteoglycans / metabolism
  • Tumor Cells, Cultured

Substances

  • Drug Combinations
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen
  • Endopeptidases