Primary structure and functional expression of a cyclic nucleotide-gated channel from rabbit aorta

M Biel; W Altenhofen; R Hullin; J Ludwig; M Freichel; V Flockerzi; N Dascal; U B Kaupp; F Hofmann

doi:10.1016/0014-5793(93)80209-d

Primary structure and functional expression of a cyclic nucleotide-gated channel from rabbit aorta

FEBS Lett. 1993 Aug 23;329(1-2):134-8. doi: 10.1016/0014-5793(93)80209-d.

Authors

M Biel¹, W Altenhofen, R Hullin, J Ludwig, M Freichel, V Flockerzi, N Dascal, U B Kaupp, F Hofmann

Affiliation

¹ Institut für Pharmakologie und Toxikologie, Technischen Universität München, Germany.

PMID: 7689061
DOI: 10.1016/0014-5793(93)80209-d

Abstract

Sequences specific for cyclic nucleotide-gated channels (CNG channels) have been amplified by PCR from cDNA of heart, aorta, sino-atrial node, cerebellum, C-cells and kidney. The complete amino acid sequence of a CNG channel from rabbit aorta has been deduced by cloning and sequence analysis of the cDNA. Synthetic RNA derived from this cDNA induces the formation of a functional CNG channel in Xenopus oocytes.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Aorta / chemistry*
Base Sequence
Cattle
Codon
Cyclic AMP / pharmacology*
Cyclic GMP / pharmacology*
DNA / chemistry
DNA / genetics
Electrophysiology
Gene Expression*
Humans
Ion Channel Gating / drug effects*
Ion Channels / chemistry*
Ion Channels / genetics
Ion Channels / physiology
Molecular Sequence Data
Open Reading Frames
Polymerase Chain Reaction
Rabbits
Sequence Homology, Amino Acid

Substances

Codon
Ion Channels
DNA
Cyclic AMP
Cyclic GMP