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Am J Clin Pathol. 1993 Jun;99(6):702-4.

Detection of antibody to hepatitis C virus by second-generation enzyme immunoassay.

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  • 1Laboratory for Viral Hepatitis, Bureau of Microbiology, Laboratory Centre for Disease Control, Ottawa, Ontario, Canada.


Of 106 samples positive for antibody to hepatitis C virus (anti-HCV) by first-generation enzyme immunoassay (EIA-1), 63 (59%), 67 (63%) and 43 (41%) were reactive by second-generation EIAs (EIA-2). Among positive EIA-1 samples, 33% were reactive by second-generation recombinant immunoblot assay (RIBA-2). A higher percentage of positive EIA-2 samples, however, were reactive by RIBA-2. In the high-risk group there was a correlation between the EIA-2 and RIBA-2 results. In the low-risk group, however, the correlation was poor except in blood donors, in whom 57% of samples were reactive by EIA-2 and RIBA-2. Results showed that the sensitivity and specificity of EIA-2s has improved although further improvement is desirable.

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