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Nucleic Acids Res. 1993 May 11;21(9):2217-21.

The exon trapping assay partly discriminates against alternatively spliced exons.

Author information

  • 1Department of Neurology (Neuroscience), Harvard Medical School, Boston, MA.

Abstract

A cosmid containing eight exons of the gene coding for the microtubule-associated tau protein was subjected to the exon trapping assay. All the constitutive exons contained in the cosmid (4, 5, 7 and 9) were efficiently captured regardless of size. Of the four alternatively spliced exons, three (3, 4A and 8) were not isolated by the assay, but the behavior of exon 6 depended on the identity of its flanking exons.

PMID:
7684835
[PubMed - indexed for MEDLINE]
PMCID:
PMC309487
Free PMC Article
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