Nerve growth factor (NGF) has been well-characterized with respect to its role as a trophic agent for various peripheral nervous system (PNS) and central nervous system (CNS) neuronal populations. Recent evidence indicates that NGF may also play a functional role in endocrine systems, although investigations in this field are only beginning to define sites of action and molecular mechanisms involved in NGF-endocrine interactions. A potential site for such an interaction to occur is within the pituitary. Previous investigations have demonstrated the presence of NGF and NGF receptors in the pituitary and our group has recently reported the presence of NGF-like immunoreactivity exclusively within the thyrotrophic cells of the anterior pituitary of the adult rat. Since many questions regarding how NGF interacts in the anterior pituitary will be more efficiently addressed using an in vitro system, it was necessary to first determine if cultured adult anterior pituitary cells retain the NGF-like staining and unique association of NGF with thyroid-stimulating hormone-producing cells seen in vivo. Results of the present investigation confirm that cultured anterior pituitary cells retain the characteristics previously observed in vivo and further demonstrate the stability of these cells and their specific NGF and pituitary hormone contents in culture for as long as 6 days.