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Trends Biochem Sci. 1995 Jul;20(7):285-6.

Purification of His-Tag fusion proteins from Escherichia coli.

Author information

  • National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702-1201, USA.

Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protein from Escherichia coli found when histidine-tagged fusion proteins are purified by metal chelate affinity chromatography. For details on how to partake in the newsgroup, see the accompanying box.

PMID:
7667882
[PubMed - indexed for MEDLINE]
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