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Cloning of the cDNA encoding human C/EBP gamma, a protein binding to the PRE-I enhancer element of the human interleukin-4 promoter.
Tumor Immunology Program, German Cancer Research Center, Heidelberg.
The positive regulatory element-I (PRE-I) is a strong enhancer element essential for expression of the human interleukin-4 (IL-4)-encoding gene. In order to identify transcription factors binding to PRE-I, we screened a cDNA expression library from human Jurkat T-cells. A cDNA encoding the human CCAAT/enhancer binding protein-gamma (hC/EBP gamma) was cloned. The deduced amino acid (aa) sequence of HC/EBP gamma contains 150 aa with high homology to mouse Ig/EBP-1 and rat C/EBP gamma. The mRNA of hC/EBP gamma is expressed at a high level in Jurkat T-cells in three forms generated via differential polyadenylation. DNA-binding experiments with recombinant protein produced in bacteria demonstrate that hC/EBP gamma binds to PRE-I, but not to unrelated DNA fragments. Our data also show that hC/EBP gamma may cooperate with Fos to bind PRE-I.
PMID: 7665092 [PubMed - indexed for MEDLINE]
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Cited by 5 PubMed Central articles
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Use of cDNA tiling arrays for identifying protein interactions selected by in vitro display technologies.
Horisawa K, Doi N, Yanagawa H.
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[PLoS One. 2008]
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Construction and validation of a Bovine Innate Immune Microarray.
Donaldson L, Vuocolo T, Gray C, Strandberg Y, Reverter A, McWilliam S, Wang Y, Byrne K, Tellam R.
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[BMC Genomics. 2005]
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[Immunology. 2000]
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