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Biochim Biophys Acta. 1995 Aug 31;1268(2):209-13.

Rab and rho GTPases are involved in specific response of periodontal ligament fibroblasts to mechanical stretching.

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  • 1Poliklinik für Kieferorthopädie, Universität Heidelberg, Germany.


To investigate the contribution of ras-related signalling molecules to the mechanotransduction process, stretch-sensing human periodontal ligament (PDL) fibroblasts were isolated and cultured in dishes with a flexible bottom. The cells were stimulated by stretching the bottom of the dishes and membrane fractions were prepared and analysed at the level of mapping small GTP-binding proteins by high resolution two-dimensional gel electrophoresis followed by renaturing transfer and an [alpha-32P]GTP-overlay procedure. This analysis revealed that mechanically-stretched PDL fibroblasts exhibit complete down-regulation of rhoA and induction of rab6, rab17 and a putative member of the rab3 subfamily in a cell type-specific manner.

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