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1: Bioorg Khim. 1995 May;21(5):359-64.Links

[Chemico-enzymatic synthesis, cloning and expression of a gene for an analog of human anaphylatoxin C5a]

[Article in Russian]

Babkina IN, Seregin SV, Daniliuk NK, Siniakov AN, Gladkova SE, Pozdniakov SG.

Chemical-enzymatic synthesis and cloning of a gene for a human anaphylatoxin C5a analog were carried out. Recombinant plasmid pRC5a providing the expression of the synthetic gene in the Escherichia coli cells was obtained. The biological activity of the expression product was demonstrated by the chemotaxis activity test and by the release of myeloperoxidases from rat peritoneal cells that was induced by bacterial cell lysates containing the recombinant protein.

PMID: 7661861 [PubMed - indexed for MEDLINE]