Abstract
The activity of the yeast activator HAP1 in vivo requires heme. A heme responsive domain of HAP1 was identified previously. It is adjacent to the DNA-binding domain and appears to block DNA binding in the absence of heme. Here we describe a novel genetic selection which yielded mutants of HAP1 that are independent of heme when assayed for activation. These mutants define a second region of HAP1, close to the activation domain, which also controls its response to heme.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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5-Aminolevulinate Synthetase / genetics
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Amino Acid Sequence
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DNA, Fungal / metabolism*
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Gene Expression Regulation, Fungal / genetics
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Heme / metabolism
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Heme / pharmacology*
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Molecular Sequence Data
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Mutagenesis
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Trans-Activators / chemistry
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription Factors
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Transcriptional Activation
Substances
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DNA, Fungal
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DNA-Binding Proteins
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Fungal Proteins
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HAP1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Trans-Activators
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Transcription Factors
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Heme
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5-Aminolevulinate Synthetase