A single expression system for the display, purification and conjugation of single-chain antibodies

N Hayashi; S Kipriyanov; P Fuchs; M Welschof; H Dörsam; M Little

doi:10.1016/0378-1119(95)00218-u

A single expression system for the display, purification and conjugation of single-chain antibodies

Gene. 1995 Jul 4;160(1):129-30. doi: 10.1016/0378-1119(95)00218-u.

Authors

N Hayashi¹, S Kipriyanov, P Fuchs, M Welschof, H Dörsam, M Little

Affiliation

¹ Recombinant Antibody Group, German Cancer Research Center, Heidelberg.

PMID: 7628706
DOI: 10.1016/0378-1119(95)00218-u

Abstract

To facilitate the purification and conjugation of single-chain antibodies (scFv) selected from a phage display library, we have incorporated His6, an amber stop codon and a C-terminal Cys into a surface expression vector. The vector also contains a lacIq gene for improving the efficiency of regulation and a sequence coding for a marker peptide.

MeSH terms

Amino Acid Sequence
Animals
Antibodies / isolation & purification
Antibodies, Monoclonal
Base Sequence
Cloning, Molecular / methods
Coliphages
DNA Primers
Enzyme-Linked Immunosorbent Assay
Gene Expression*
Genes, Immunoglobulin*
Mice
Molecular Sequence Data
Plasmids
Polymerase Chain Reaction
Recombinant Fusion Proteins / biosynthesis*
Recombinant Fusion Proteins / isolation & purification
Restriction Mapping

Substances

Antibodies
Antibodies, Monoclonal
DNA Primers
Recombinant Fusion Proteins

Associated data

GENBANK/X82190